Modeling of femoral head aseptic necrosis in Wistar rats

Aim. To develop an animal model of femoral head aseptic necrosis for studying Legg–Calvé–Perthes disease. Materials and Methods. induce the development necrosis, we used Wistar rats (n = 8) which suffered from combined hypoperfusion increased intra-articular pressure in hip joint. Having employed isoflurane anesthesia, performed incision (≈ 3 cm length) on outer surface thigh projection joint then excised periosteum proximal third femur. A dense vicryl ligature was applied around neck to reduce blood perfusion head. Further, 1.5 mL 2% rheopolyglucinum solution (10% isotonic dextran, 30-40 kDa molecular weight) injected into cavity increase pressure. Rats were sacrificed upon 8-week follow-up with subsequent X-ray histological examination. Results. Our includes two main components disease: insufficient In all (8/8) cases, achieved. Eight weeks post intervention, condition femur 8 similar impression fracture. Conclusion. fully reflects pathogenesis LeggCalve-Perthes disease can be therefore experimental studies.